pCDH_Str-KDEL_ManII-SBP-EGFP
(Plasmid #65258)

• Purpose: synchronize trafficking of ManII from the ER (RUSH system)
• Depositing Lab: Franck Perez
• Publication: Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.

Backbone

• Vector backbone: pCDH-CMV-MCS-EF1-puro
• Backbone manufacturer: system biosciences
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Streptavidin-KDEL and truncated Mannosidase II
• Alt name: MAN2A1
• Species: M. musculus (mouse)
• Mutation: contains only amino acids 1 to 116 of ManII
• Promoter: CMV
• Tags / Fusion Proteins:
  • Streptavidin Binding Protein (SBP) (C terminal on insert)
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: IRES-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

luminal tagging

A350S mutation in EGFP, depositor states that this mutation does not affect plasmid function.