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pLXSN-FLK1 TM
(Plasmid #65249)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLXSN
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 8300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLK1 TM
  • Alt name
    VEGFR2 TM; KDR TM
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Mutation
    deleted AA 786-1346
  • GenBank ID
    NM_010612
  • Entrez Gene
    Kdr (a.k.a. 6130401C07, Flk-1, Flk1, Krd-1, Ly73, VEGFR-2, VEGFR2, orv, sVEGFR-2)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer CTCTCCCCCTTGAACCTC
  • 3′ sequencing primer GACTTTCCACACCCTAACTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert codes for extracellular and transmembrane domains of FLK1. Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLXSN-FLK1 TM was a gift from Axel Ullrich (Addgene plasmid # 65249 ; http://n2t.net/addgene:65249 ; RRID:Addgene_65249)

  • For your References section:

    Glioblastoma growth inhibited in vivo by a dominant-negative Flk-1 mutant. Millauer B, Shawver LK, Plate KH, Risau W, Ullrich A. Nature. 1994 Feb 10;367(6463):576-9. 10.1038/367576a0 PubMed 8107827
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