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Addgene

pcDNA3.1 mCherry Gamma 3
(Plasmid #65234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6370
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mcherry
  • Insert Size (bp)
    717
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Gng3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    228
  • Entrez Gene
    Gng3
  • Promoter CMV
  • Tag / Fusion Protein
    • 6-His tag (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 mCherry Gamma 3 was a gift from Narasimhan Gautam (Addgene plasmid # 65234 ; http://n2t.net/addgene:65234 ; RRID:Addgene_65234)

  • For your References section:

    A G-protein subunit translocation embedded network motif underlies GPCR regulation of calcium oscillations. Giri L, Patel AK, Karunarathne WK, Kalyanaraman V, Venkatesh KV, Gautam N. Biophys J. 2014 Jul 1;107(1):242-54. doi: 10.1016/j.bpj.2014.05.020. 10.1016/j.bpj.2014.05.020 PubMed 24988358
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