pcDNA3.1 mCherry Gamma 3
(Plasmid #65234)

• Purpose: To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell
• Depositing Lab: Narasimhan Gautam
• Publication: Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi: 10.1016/j.bpj.2014.05.020.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6370
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mcherry
• Insert Size (bp): 717
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH reverse primer

Gene/Insert 2

• Gene/Insert name: Gng3
• Species: M. musculus (mouse)
• Insert Size (bp): 228
• Promoter: CMV
• Tag / Fusion Protein:
  • 6-His tag (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (destroyed during cloning)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry