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pMOS003-lenti-CMV-GCaMPer
(Plasmid #65227)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FCK
  • Backbone manufacturer
    Pavel Osten
  • Backbone size w/o insert (bp) 8500
  • Total vector size (bp) 9981
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GCaMPer
  • Alt name
    ER-targeted low affinity GCaMP3 variant 10.19
  • Species
    Synthetic
  • Insert Size (bp)
    1455
  • Mutation
    GCaMP3 (D324G, D360G, D397G, D435G)
  • Promoter CMV
  • Tag / Fusion Protein
    • circularly permuted GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mark Henderson and Brandon Harvey at NIDA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMOS003-lenti-CMV-GCaMPer was a gift from Adam Cohen & Brandon Harvey (Addgene plasmid # 65227 ; http://n2t.net/addgene:65227 ; RRID:Addgene_65227)

  • For your References section:

    A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic Reticulum Calcium Store. Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X, Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK. PLoS One. 2015 Oct 9;10(10):e0139273. doi: 10.1371/journal.pone.0139273. eCollection 2015. PONE-D-15-20579 [pii] PubMed 26451944
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