GFP-ESR2
(Plasmid
#65212)
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PurposeFull length mammalian expression vector for hESR2
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65212 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAcGFP1-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameESR2
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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Entrez GeneESR2 (a.k.a. ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8)
- Promoter CMV
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Tag
/ Fusion Protein
- AcGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer sREACh-F (gaccccaacgagaagcgcg)
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-ESR2 was a gift from Ratna Vadlamudi (Addgene plasmid # 65212 ; http://n2t.net/addgene:65212 ; RRID:Addgene_65212)