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Addgene

pEBG-PELP1
(Plasmid #65209)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65209 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBG
  • Backbone manufacturer
    Supplied by Addgene
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 9400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PELP1
  • Alt name
    proline, glutamate and leucine rich protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3400
  • Entrez Gene
    PELP1 (a.k.a. MNAR, P160)
  • Promoter EF1a
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EF1aF
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PELP1 cDNA has internal BamHI site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG-PELP1 was a gift from Ratna Vadlamudi (Addgene plasmid # 65209 ; http://n2t.net/addgene:65209 ; RRID:Addgene_65209)

  • For your References section:

    Cyclin-dependent kinase-mediated phosphorylation plays a critical role in the oncogenic functions of PELP1. Nair BC, Nair SS, Chakravarty D, Challa R, Manavathi B, Yew PR, Kumar R, Tekmal RR, Vadlamudi RK. Cancer Res. 2010 Sep 15;70(18):7166-75. doi: 10.1158/0008-5472.CAN-10-0628. Epub 2010 Aug 31. 10.1158/0008-5472.CAN-10-0628 PubMed 20807815
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