pGEM5Z(+)-EGFP
(Plasmid #65206)

• Purpose: EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
• Depositing Lab: LuZhe Sun
• Publication: Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. doi: 10.1016/j.ab.2009.02.020. Epub 2009 Feb 25.

Backbone

• Vector backbone: pGEM5Z(+)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3001
• Total vector size (bp): 4604
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 1632
• Mutation: Restriction enzyme recognition sites added either side of the EGFP start codon (EcoRV upstream, NruI downstream)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: EcoRV (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned the CMV-EGFP cassette from pEGFP-N1 (Clontech) into pGEM5Z(+) (Promega)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To be used in conjunction with p189: www.addgene.org/65207. See corrigendum/erratum for original publication (https://doi.org/10.1016/j.ab.2009.10.024) before performing experiments with this plasmid.