Skip to main content
Addgene

pYTK035
(Plasmid #65142)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pYTK001
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    I-SceI (ORF)
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYTK035 was a gift from John Dueber (Addgene plasmid # 65142 ; http://n2t.net/addgene:65142 ; RRID:Addgene_65142)

  • For your References section:

    A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. Lee ME, DeLoache WC, Cervantes B, Dueber JE. ACS Synth Biol. 2015 Apr 14. 10.1021/sb500366v PubMed 25871405
Included in kit:
Related items:
Commonly requested with: