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Addgene

zebrafish SLC2A2 (zfSLC2A2)
(Plasmid #65103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBK-CMV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC2A2
  • Alt name
    GLUT2 (Glucose transporter 2)
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2500
  • GenBank ID
    DQ098687
  • Entrez Gene
    slc2a2 (a.k.a. glut2, wu:fb62h10)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    zebrafish SLC2A2 (zfSLC2A2) was a gift from Josep Planas (Addgene plasmid # 65103 ; http://n2t.net/addgene:65103 ; RRID:Addgene_65103)

  • For your References section:

    Evolutionary structural and functional conservation of an ortholog of the GLUT2 glucose transporter gene (SLC2A2) in zebrafish. Castillo J, Crespo D, Capilla E, Diaz M, Chauvigne F, Cerda J, Planas JV. Am J Physiol Regul Integr Comp Physiol. 2009 Nov;297(5):R1570-81. doi: 10.1152/ajpregu.00430.2009. Epub 2009 Sep 23. 10.1152/ajpregu.00430.2009 PubMed 19776250
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