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pGL3-fugu SLC2A4 promoter
(Plasmid #65101)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC2A4 promoter
  • Alt name
    GLUT4 promoter
  • Species
    Fugu (Takifugu rubripes)
  • Insert Size (bp)
    1300

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer 3
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is also known as pGL3-trSLC2A4P.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-fugu SLC2A4 promoter was a gift from Josep Planas (Addgene plasmid # 65101 ; http://n2t.net/addgene:65101 ; RRID:Addgene_65101)

  • For your References section:

    Mechanisms regulating GLUT4 transcription in skeletal muscle cells are highly conserved across vertebrates. Marin-Juez R, Diaz M, Morata J, Planas JV. PLoS One. 2013 Nov 18;8(11):e80628. doi: 10.1371/journal.pone.0080628. eCollection 2013. PONE-D-13-30467 [pii] PubMed 24260440
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