pGL3-sbIL6P
(Plasmid
#65099)
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PurposeLuciferase reporter construct under the seabream IL6 promoter.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4800
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameinterleukin-6 promoter
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Alt nameIL-6
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SpeciesSeabream (Sparus aurata)
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Insert Size (bp)1100
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer RVprimer 3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-sbIL6P was a gift from Josep Planas (Addgene plasmid # 65099 ; http://n2t.net/addgene:65099 ; RRID:Addgene_65099) -
For your References section:
Transcriptional regulation of the gilthead seabream (Sparus aurata) interleukin-6 gene promoter. Castellana B, Marin-Juez R, Planas JV. Fish Shellfish Immunol. 2013 Jul;35(1):71-8. doi: 10.1016/j.fsi.2013.04.012. Epub 2013 Apr 18. 10.1016/j.fsi.2013.04.012 PubMed 23602849