pBAD33-Gm
(Plasmid #65098)

• Purpose: (Empty Backbone) bacterial expression vector with Gentamycin selection, based on pBAD33
• Depositing Lab: Juan Tomas
• Publication: Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. doi: 10.1128/JB.01395-08. Epub 2009 Jan 16.

Backbone

• Vector backbone: pBAD33
• Backbone manufacturer: Beckwith Lab
• Modifications to backbone: the gentamicin resistance gene (aacC1) of the pUCGmlox vector was used. pUCGmlox was digested with SmaI, and the fragment containing the lox-flanked aacC1 gene was purified and cloned into the PvuII and ScaI sites in previously digested pBAD33, replacing its chloramphenicol resistance gene (cat)
• Vector type: Bacterial Expression
• Promoter: pBAD

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: grow in 10ug/ul Gentamycin. Too much gentamycin is toxic for e. coli.
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBad-Fwd
• 3′ sequencing primer: pBad-Rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry