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Addgene

shCCL20
(Plasmid #65096)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSM2C
  • Backbone manufacturer
    Greg Hannon (PMID 16200065)
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ccl20 shRNA
  • gRNA/shRNA sequence
    5'-AAGCAGAACTGGGTGAAAATAGTGAAGCCACAGATGTATTTTCACCCAGTTCTGCTT-3'
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ccl20 (a.k.a. CKb4, LARC, MIP-3A, MIP-3[a], MIP3A, ST38, Scya20, exodus-1)
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    shCCL20 was a gift from Pau Sancho-Bru (Addgene plasmid # 65096 ; http://n2t.net/addgene:65096 ; RRID:Addgene_65096)

  • For your References section:

    CCL20 mediates lipopolysaccharide induced liver injury and is a potential driver of inflammation and fibrosis in alcoholic hepatitis. Affo S, Morales-Ibanez O, Rodrigo-Torres D, Altamirano J, Blaya D, Dapito DH, Millan C, Coll M, Caviglia JM, Arroyo V, Caballeria J, Schwabe RF, Gines P, Bataller R, Sancho-Bru P. Gut. 2014 Nov;63(11):1782-92. doi: 10.1136/gutjnl-2013-306098. Epub 2014 Jan 10. 10.1136/gutjnl-2013-306098 PubMed 24415562
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