pFRT-TODestFLAGHA_RBPMS_KDR_F65A
(Plasmid
#65092)
-
PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generation
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 65092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA5/FRT/TO
-
Backbone manufacturerLife Technologies, Modified by Tuschl lab
- Backbone size w/o insert (bp) 5372
-
Vector typeMammalian Expression
-
Selectable markersHygromycin, Blasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameRBPMS
-
SpeciesH. sapiens (human)
-
Insert Size (bp)633
-
Mutationchange phenylalanine 65 to alanine (KDR backbone)
-
GenBank IDNM_001008710
-
Entrez GeneRBPMS (a.k.a. HERMES)
- Promoter CMV, 2x Tet
-
Tag
/ Fusion Protein
- FLAG HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFRT-TODestFLAGHA_RBPMS_KDR_F65A was a gift from Thomas Tuschl (Addgene plasmid # 65092 ; http://n2t.net/addgene:65092 ; RRID:Addgene_65092) -
For your References section:
Structural basis underlying CAC RNA recognition by the RRM domain of dimeric RNA-binding protein RBPMS. Teplova M, Farazi TA, Tuschl T, Patel DJ. Q Rev Biophys. 2015 Sep 8:1-11. 10.1017/S0033583515000207 PubMed 26347403