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Addgene

RhoC FLARE.sc mCer, mVenus - wt
(Plasmid #65071)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65071 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 7888
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBD, mCerulean, mVenus, RhoC
  • Insert Size (bp)
    2688
  • Mutation
    T153M in mVenus
  • Promoter CMV, p10

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RhoC FLARE.sc mCer, mVenus - wt was a gift from Klaus Hahn (Addgene plasmid # 65071 ; http://n2t.net/addgene:65071 ; RRID:Addgene_65071)

  • For your References section:

    A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L. PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013. PONE-D-13-18183 [pii] PubMed 24224016
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