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pMSCV-flag-myr-Akt1-IRES-GFP
(Plasmid #65063)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 8161
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Akt1
  • Alt name
    pkb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1661
  • Entrez Gene
    AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Tags / Fusion Proteins
    • myristoylation tag (N terminal on insert)
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer MSCV LTR 5'
  • 3′ sequencing primer MSCV LTR 3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    insert cDNA was from Jean Zhao at Dana-Farber Cancer Institute
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the plasmid is the property of Dana-Farber Cancer Institute

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-flag-myr-Akt1-IRES-GFP was a gift from Kira Gritsman & Jean Zhao (Addgene plasmid # 65063 ; http://n2t.net/addgene:65063 ; RRID:Addgene_65063)

  • For your References section:

    Constitutively active AKT depletes hematopoietic stem cells and induces leukemia in mice. Kharas MG, Okabe R, Ganis JJ, Gozo M, Khandan T, Paktinat M, Gilliland DG, Gritsman K. Blood. 2010 Feb 18;115(7):1406-15. doi: 10.1182/blood-2009-06-229443. Epub 2009 Dec 14. 10.1182/blood-2009-06-229443 PubMed 20008787
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