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Addgene

pWPI-NP1
(Plasmid #65060)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWPI
  • Backbone manufacturer
    Didier Trono
  • Backbone size w/o insert (bp) 11078
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuronal Pentraxin 1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1302
  • Mutation
    * see comments
  • GenBank ID
    NM_153735.2
  • Entrez Gene
    Nptx1
  • Promoter EF1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmeI (destroyed during cloning)
  • 3′ cloning site PmeI (destroyed during cloning)
  • 5′ sequencing primer EF1a-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

* N280D and R322Q are present in NP1 and this plasmid should function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPI-NP1 was a gift from Ramon Trullas (Addgene plasmid # 65060 ; http://n2t.net/addgene:65060 ; RRID:Addgene_65060)

  • For your References section:

    Neuronal pentraxin 1 negatively regulates excitatory synapse density and synaptic plasticity. Figueiro-Silva J, Gruart A, Clayton KB, Podlesniy P, Abad MA, Gasull X, Delgado-Garcia JM, Trullas R. J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 10.1523/JNEUROSCI.2548-14.2015. 10.1523/JNEUROSCI.2548-14.2015 PubMed 25855168
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