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Addgene

pCMS-eGFP-RTP801
(Plasmid #65057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMS-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDIT4
  • Alt name
    RTP801
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    690
  • GenBank ID
    140942
  • Entrez Gene
    Ddit4 (a.k.a. Rtp801)
  • Promoter CMV-IE
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are 2 mutations if compared to PubMed DDIT4 R. Norvegicus sequence (L121V and S208G) because the insert was cloned out of DC12 cells DNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMS-eGFP-RTP801 was a gift from Lloyd Greene & Cristina Malagelada (Addgene plasmid # 65057 ; http://n2t.net/addgene:65057 ; RRID:Addgene_65057)

  • For your References section:

    RTP801 is elevated in Parkinson brain substantia nigral neurons and mediates death in cellular models of Parkinson's disease by a mechanism involving mammalian target of rapamycin inactivation. Malagelada C, Ryu EJ, Biswas SC, Jackson-Lewis V, Greene LA. J Neurosci. 2006 Sep 27;26(39):9996-10005. 10.1523/JNEUROSCI.3292-06.2006 PubMed 17005863
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