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Addgene

p-miR-SC
(Plasmid #65055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5175
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsa-miR-148a (mutated)
  • Alt name
    MIR148A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    335
  • GenBank ID
    406940 (mutated)
  • Entrez Gene
    MIR148A (a.k.a. MIRN148, MIRN148A, hsa-mir-148, mir-148a)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBABE-5
  • 3′ sequencing primer pBABE-3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid was also described in PubMed ID 25714032.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-miR-SC was a gift from Cristina Fillat (Addgene plasmid # 65055 ; http://n2t.net/addgene:65055 ; RRID:Addgene_65055)

  • For your References section:

    miR-148a- and miR-216a-regulated oncolytic adenoviruses targeting pancreatic tumors attenuate tissue damage without perturbation of miRNA activity. Bofill-De Ros X, Gironella M, Fillat C. Mol Ther. 2014 Sep;22(9):1665-77. doi: 10.1038/mt.2014.98. Epub 2014 Jun 4. 10.1038/mt.2014.98 PubMed 24895996
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