pluc-miR148a-148aT
(Plasmid #65053)

• Purpose: Reporter plasmid to detect miR148/152 family expression
• Depositing Lab: Cristina Fillat
• Publication: Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 10.1038/mt.2014.98. Epub 2014 Jun 4.

Backbone

• Vector backbone: pGL4.13
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4637
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Engineered target sites miR-148a-3p
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 240
• Entrez Gene: MIR148A (a.k.a. MIRN148, MIRN148A, hsa-mir-148, mir-148a)
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (to EcoRI) (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • GenBank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry