Mouse GFP-PRMT7 LDIG(69-72)AAAA
(Plasmid #65044)

• Purpose: Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant
• Depositing Lab: Steven Clarke
• Publication: Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-25. doi: 10.1074/jbc.M113.525345. Epub 2013 Nov 18.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6779
• Vector type: Mammalian Expression ; Bacterial Propagation
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PRMT7
• Alt name: Protein Arginine Methyltransferase 7
• Species: M. musculus (mouse)
• Insert Size (bp): 2079
• Mutation: L69A D70A I71A G72A
• Entrez Gene: Prmt7 (a.k.a. 4933402B05Rik, BC006705)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mark Bedford, University of Texas MD Anderson

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results found an F4S amino acid change in the PRMT7 translation relative to genbank ID NM_145404.1.