pUC18T-mini-Tn7T-Zeo-dsRedExpress
(Plasmid
#65040)
-
PurposeZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 65040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
* Log in to view industry pricing.
Backbone
-
Vector backbonepUC18T-mini-Tn7T-Zeo
-
Backbone manufacturerHerbert Schweizer
- Total vector size (bp) 5377
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Bleocin (Zeocin), 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namedsRedExpress
-
Alt nameRFP
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank Accession # DQ493888
Please note that Addgene's sequencing results (and the updated full plasmid sequence listed above) differ from the GenBank sequence. The effect of these differences is unknown.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pUC18T-mini-Tn7T-Zeo-dsRedExpress was a gift from Herbert Schweizer (Addgene plasmid # 65040 ; http://n2t.net/addgene:65040 ; RRID:Addgene_65040) -
For your References section:
mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Choi KH, Schweizer HP. Nat Protoc. 2006;1(1):153-61. 10.1038/nprot.2006.24 PubMed 17406227