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Addgene

pUC18T-mini-Tn7T-Zeo-gfpmut3
(Plasmid #65037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18T-mini-Tn7T-Zeo
  • Backbone manufacturer
    Herbert Schweizer
  • Total vector size (bp) 5437
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Bleocin (Zeocin), 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gfpmut3
  • Alt name
    GFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank Accession # DQ493885

Please note that Addgene's sequencing results (and the updated full plasmid sequence listed above) differ from the GenBank sequence, including a R80Q mutation in gfpmut3. The effect of these differences is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18T-mini-Tn7T-Zeo-gfpmut3 was a gift from Herbert Schweizer (Addgene plasmid # 65037 ; http://n2t.net/addgene:65037 ; RRID:Addgene_65037)

  • For your References section:

    mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Choi KH, Schweizer HP. Nat Protoc. 2006;1(1):153-61. 10.1038/nprot.2006.24 PubMed 17406227
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