pUC18T-mini-Tn7T-Tp-eyfp
(Plasmid
#65035)
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PurposeTpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18T-mini-Tn7T-Tp
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Backbone manufacturerHerbert Schweizer
- Total vector size (bp) 5707
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsalso Trimethoprim resistant
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeyfp
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Alt nameYFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank Accession # DQ493883
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC18T-mini-Tn7T-Tp-eyfp was a gift from Herbert Schweizer (Addgene plasmid # 65035 ; http://n2t.net/addgene:65035 ; RRID:Addgene_65035) -
For your References section:
mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Choi KH, Schweizer HP. Nat Protoc. 2006;1(1):153-61. 10.1038/nprot.2006.24 PubMed 17406227