pUC18-mini-Tn7T-Gm-LacZ20
(Plasmid
#65027)
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Purpose(Empty Backbone) GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUC18-mini-Tn7T-Gm
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Backbone manufacturerHerbert Schweizer
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- lacZ (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsalso Ampicillin resistant
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank Accession # DQ296495
Compared to the Genbank reference sequence DQ296495, Addgene's sequencing results found T920I, D999E and F1000L mutations in lacZ. The effect of these differences is unknown. Note that the amino acid numbering is relative to the lacZ encoded by this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC18-mini-Tn7T-Gm-LacZ20 was a gift from Herbert Schweizer (Addgene plasmid # 65027 ; http://n2t.net/addgene:65027 ; RRID:Addgene_65027) -
For your References section:
mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Choi KH, Schweizer HP. Nat Protoc. 2006;1(1):153-61. 10.1038/nprot.2006.24 PubMed 17406227
