pUC18-mini-Tn7T-Gm-LacZ10
(Plasmid #65026)

• Purpose: (Empty Backbone) GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.
• Depositing Lab: Herbert Schweizer
• Publication: Choi et al Nat Protoc. 2006;1(1):153-61.

Backbone

• Vector backbone: pUC18-mini-Tn7T-Gm
• Backbone manufacturer: Herbert Schweizer
• Vector type: Bacterial Expression
• Tag / Fusion Protein:
  • lacZ (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: also Ampicillin resistant
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • DQ296494
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

GenBank Accession # DQ296494

Compared to the Genbank reference sequence DQ296494, Addgene's sequencing results found T920I, D999E and F1000L mutations in lacZ. The effect of these differences is unknown. Note that the amino acid numbering is relative to the lacZ encoded by this plasmid.