pAAV-CaMKII-Jaws-KGC-GFP-ER2
(Plasmid #65015)

• Purpose: AAV-mediated expression of Jaws under the CaMKII promoter
• Depositing Lab: Edward Boyden
• Publication: Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014 Jul 6.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5107
• Total vector size (bp): 6740
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Please note that this backbone is highly recombination prone and should be amplified in a rec- strain like Sure2 or Stbl3, and grown using carbenicillin rather than ampicillin.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Jaws-KGC-GFP-ER2
• Alt name: Jaws
• Species: Haloarcula salinarum (strain Shark)
• Insert Size (bp): 1650
• Mutation: K200R W214F
• GenBank ID: KM000926.1
• Promoter: CaMKII
• Tags / Fusion Proteins:
  • KGC (C terminal on insert)
  • GFP (C terminal on insert)
  • ER2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CAC ACA GTC CTG CAG TAT TGT GTA TAT
• 3′ sequencing primer: CAA GGA GGA GAA AAT GAA AGC C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry