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fck-Jaws-KGC-GFP-ER2
(Plasmid #65012)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65012 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FCK(1.3)GW
  • Backbone manufacturer
    Pavel Osten
  • Backbone size w/o insert (bp) 9226
  • Total vector size (bp) 10900
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Please note that this backbone is highly recombination prone and should be amplified in a rec- strain like Sure2 or Stbl3, using carbenicillin rather than ampicillin.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jaws-KGC-GFP-ER2
  • Alt name
    Jaws
  • Species
    Haloarcula salinarum (strain Shark)
  • Insert Size (bp)
    1656
  • Mutation
    K200R W214F
  • GenBank ID
    KM000926.1
  • Promoter CaMKII
  • Tags / Fusion Proteins
    • KGC (C terminal on insert)
    • GFP (C terminal on insert)
    • ER2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CAC ACA GTC CTG CAG TAT TGT GTA TAT
  • 3′ sequencing primer CAA GGA GGA GAA AAT GAA AGC C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    fck-Jaws-KGC-GFP-ER2 was a gift from Edward Boyden (Addgene plasmid # 65012 ; http://n2t.net/addgene:65012 ; RRID:Addgene_65012)

  • For your References section:

    Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y, Tye KM, Roska B, Cardin JA, Boyden ES. Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014 Jul 6. 10.1038/nn.3752 PubMed 24997763
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