pCRISPReporter-mCherry
(Plasmid #65008)

• Purpose: E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
• Depositing Lab: Mattheos Koffas
• Publication: Cress et al ACS Synth Biol. 2015 Mar 30.

Backbone

• Vector backbone: pCRISPReporter (pETM6/pET-Duet-1)
• Backbone size w/o insert (bp): 4343
• Total vector size (bp): 5011
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic; Codon-optimized for E. coli expression
• Insert Size (bp): 708
• Promoter: User-defined

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: GGGCCCGCTGGGAGTTCGTAG
• 3′ sequencing primer: GTCGAGGTGCCGTAAAGCACTAAATCG

Resource Information

• Supplemental Documents:
  • GenBank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry