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PurposeYeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep415TEF
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGlutaredoxin-1
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Alt nameGRX1
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- roGFP2 (N terminal on insert)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p415TEF cyto roGFP2-Grx1(yeast) was a gift from Tobias Dick (Addgene plasmid # 65004 ; http://n2t.net/addgene:65004 ; RRID:Addgene_65004) -
For your References section:
Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis. Morgan B, Ezerina D, Amoako TN, Riemer J, Seedorf M, Dick TP. Nat Chem Biol. 2013 Feb;9(2):119-25. doi: 10.1038/nchembio.1142. Epub 2012 Dec 16. 10.1038/nchembio.1142 PubMed 23242256