pCaSpeR4 mito roGFP2-Orp1
(Plasmid
#65001)
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PurposeExpression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCaSpeR4
- Total vector size (bp) 12070
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOrp1
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Alt nameGPX3_YEAST
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SpeciesS. cerevisiae (budding yeast)
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Mutationmitochondrial targeting sequence
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Entrez GeneHYR1 (a.k.a. YIR037W, GPX3, ORP1)
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Tag
/ Fusion Protein
- roGFP2 (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCaSpeR4 mito roGFP2-Orp1 was a gift from Tobias Dick (Addgene plasmid # 65001 ; http://n2t.net/addgene:65001 ; RRID:Addgene_65001) -
For your References section:
In vivo mapping of hydrogen peroxide and oxidized glutathione reveals chemical and regional specificity of redox homeostasis. Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP. Cell Metab. 2011 Dec 7;14(6):819-29. doi: 10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17. 10.1016/j.cmet.2011.10.010 PubMed 22100409