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Addgene

pCaSpeR4 mito roGFP2-Grx1
(Plasmid #65000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR4
  • Total vector size (bp) 11890
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Glutaredoxin-1
  • Alt name
    GLRX1_HUMAN
  • Species
    H. sapiens (human)
  • Mutation
    mitochondrial targeting sequence
  • Entrez Gene
    GLRX (a.k.a. GRX, GRX1)
  • Tag / Fusion Protein
    • roGFP2 (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence discrepancies found during QC should not affect function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCaSpeR4 mito roGFP2-Grx1 was a gift from Tobias Dick (Addgene plasmid # 65000 ; http://n2t.net/addgene:65000 ; RRID:Addgene_65000)

  • For your References section:

    In vivo mapping of hydrogen peroxide and oxidized glutathione reveals chemical and regional specificity of redox homeostasis. Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP. Cell Metab. 2011 Dec 7;14(6):819-29. doi: 10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17. 10.1016/j.cmet.2011.10.010 PubMed 22100409
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