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PurposeMammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLPCX
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Backbone manufacturerClontech
- Total vector size (bp) 7518
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameOrp1
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Alt nameGPX3_YEAST
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneHYR1 (a.k.a. YIR037W, GPX3, ORP1)
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Tag
/ Fusion Protein
- roGFP2 (C terminal on insert)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPCX roGFP2-Orp1 was a gift from Tobias Dick (Addgene plasmid # 64991 ; http://n2t.net/addgene:64991 ; RRID:Addgene_64991) -
For your References section:
Proximity-based protein thiol oxidation by H2O2-scavenging peroxidases. Gutscher M, Sobotta MC, Wabnitz GH, Ballikaya S, Meyer AJ, Samstag Y, Dick TP. J Biol Chem. 2009 Nov 13;284(46):31532-40. doi: 10.1074/jbc.M109.059246. Epub 2009 Sep 15. 10.1074/jbc.M109.059246 PubMed 19755417