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PurposeMammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEIGW
- Total vector size (bp) 9883
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGlutaredoxin-1
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Alt nameGLRX1_HUMAN
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SpeciesH. sapiens (human)
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Entrez GeneGLRX (a.k.a. GRX, GRX1)
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Tag
/ Fusion Protein
- roGFP2 (C terminal on insert)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sequence discrepancies found during QC should not affect function
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEIGW Grx1-roGFP2 was a gift from Tobias Dick (Addgene plasmid # 64990 ; http://n2t.net/addgene:64990 ; RRID:Addgene_64990) -
For your References section:
Real-time imaging of the intracellular glutathione redox potential. Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, Meyer AJ, Dick TP. Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008 May 11. 10.1038/nmeth.1212 PubMed 18469822