pTH1-SpSrtA
(Plasmid #64987)

• Purpose: Expresses the wild type of Streptococcus pyogenes sortase A in E. coli
• Depositing Lab: Teruyuki Nagamune
• Publication: Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585. Epub 2012 Jul 4.

Backbone

• Vector backbone: pTWIN1
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 6688
• Total vector size (bp): 7135
• Modifications to backbone: Addition of a His6 tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: S. pyogenes sortase A
• Alt name: SpSrtA
• Species: Streptococcus pyogenes M1 GAS
• Insert Size (bp): 513
• Mutation: deleted amino acids 1-81
• Promoter: T7 promoter
• Tags / Fusion Proteins:
  • Mxe GyrA intein (C terminal on backbone)
  • chitin-binding protein (C terminal on backbone)
  • His6 tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SapI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• A portion of this plasmid was derived from a plasmid made by: S. pyogenes sortase A is subcloned from a synthetic gene whose codons are optimized from E. coli expression.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry