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Addgene

pTH1-SaSrtA-KQ
(Plasmid #64982)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIN1
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6688
  • Total vector size (bp) 7135
  • Modifications to backbone
    Addition of a His6 tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sortase A
  • Alt name
    SrtA
  • Species
    Staphylococcus aureus ATCC 10832
  • Insert Size (bp)
    447
  • Mutation
    deleted amino acids 1-59, E105K, E108Q
  • Tags / Fusion Proteins
    • Mxe GyrA intein (C terminal on backbone)
    • chitin-binding protein (C terminal on backbone)
    • His6 tag (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    S. aureus sortase A is originally cloned from a genomic DNA of S. aureus (ATCC 10832D) in our lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH1-SaSrtA-KQ was a gift from Teruyuki Nagamune (Addgene plasmid # 64982 ; http://n2t.net/addgene:64982 ; RRID:Addgene_64982)

  • For your References section:

    Design of Ca2+-independent Staphylococcus aureus sortase A mutants. Hirakawa H, Ishikawa S, Nagamune T. Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585. Epub 2012 Jul 4. 10.1002/bit.24585 PubMed 22729808
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