pTH1-SaSrtA-KA
(Plasmid #64981)

• Purpose: Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli
• Depositing Lab: Teruyuki Nagamune
• Publication: Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585. Epub 2012 Jul 4.

Backbone

• Vector backbone: pTWIN1
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 6688
• Total vector size (bp): 7135
• Modifications to backbone: Addition of a His6 tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sortase A
• Alt name: SrtA
• Species: Staphylococcus aureus ATCC 10832
• Insert Size (bp): 447
• Mutation: deleted amino acids 1-59, E105K, E108A
• Tags / Fusion Proteins:
  • Mxe GyrA intein (C terminal on backbone)
  • chitin-binding protein (C terminal on backbone)
  • His6 tag (C terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: S. aureus sortase A is originally cloned from a genomic DNA of S. aureus (ATCC 10832D) in our lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry