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Addgene

pLPCX mito Grx1-roGFP2
(Plasmid #64977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPCX
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 7551
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Glutaredoxin-1
  • Alt name
    GLRX1_HUMAN
  • Species
    H. sapiens (human)
  • Mutation
    mitochondrial targeting sequence
  • Entrez Gene
    GLRX (a.k.a. GRX, GRX1)
  • Tag / Fusion Protein
    • roGFP2 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPCX mito Grx1-roGFP2 was a gift from Tobias Dick (Addgene plasmid # 64977 ; http://n2t.net/addgene:64977 ; RRID:Addgene_64977)

  • For your References section:

    Real-time imaging of the intracellular glutathione redox potential. Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, Meyer AJ, Dick TP. Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008 May 11. 10.1038/nmeth.1212 PubMed 18469822
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