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Addgene

pQE-60 roGFP2-Orp1-His
(Plasmid #64976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-60
  • Backbone manufacturer
    Qiagen
  • Total vector size (bp) 4730
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Orp1
  • Alt name
    GPX3_YEAST
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HYR1 (a.k.a. YIR037W, GPX3, ORP1)
  • Tags / Fusion Proteins
    • roGFP2 (N terminal on insert)
    • His (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE-60 roGFP2-Orp1-His was a gift from Tobias Dick (Addgene plasmid # 64976 ; http://n2t.net/addgene:64976 ; RRID:Addgene_64976)

  • For your References section:

    Proximity-based protein thiol oxidation by H2O2-scavenging peroxidases. Gutscher M, Sobotta MC, Wabnitz GH, Ballikaya S, Meyer AJ, Samstag Y, Dick TP. J Biol Chem. 2009 Nov 13;284(46):31532-40. doi: 10.1074/jbc.M109.059246. Epub 2009 Sep 15. 10.1074/jbc.M109.059246 PubMed 19755417
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