pUC18R6KT-mini-Tn7T-Km
(Plasmid #64969)

• Purpose: (Empty Backbone) mini-Tn7 base vector with transcriptional terminator and oriT
• Depositing Lab: Herbert Schweizer
• Publication: Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Backbone

• Vector backbone: pUC18R6KT-mini-Tn7T
• Backbone manufacturer: Herbert Schweizer
• Modifications to backbone: addition of FRT-flanked KmR marker
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • Annotated Genbank file
• Addgene Notes:
  • Addgene Restriction Digest
  • Plasmidsaurus Histogram
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

The uncut plasmid runs as a multimer (>10kb). Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If the monomer is needed, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.