pUC18-mini-Tn7T-Gm-lux
(Plasmid #64963)

• Purpose: mini-Tn7 luxCDABE transcriptional fusion vector
• Depositing Lab: Herbert Schweizer
• Publication: Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Backbone

• Vector backbone: pUC18-mini-Tn7T-Gm
• Backbone manufacturer: Herbert Schweizer
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: also ampicillin resistant
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lux operon

Resource Information

• Supplemental Documents:
  • AY962893
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

GenBank Accession # AY962893

Compared to the Genbank reference sequence AY962893, Addgene's sequencing results found a A85R mutation in luxB. The effect of this difference is unknown. Note that Addgene has not sequenced the entire luxCDABE fusion.