pFCM1
(Plasmid
#64948)
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Purposesource of CmR FRT cassette
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64948 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFRT2
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Backbone manufacturerHerbert Schweizer
- Total vector size (bp) 3851
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFRT-cat-FRT
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Alt namechloramphenicol acetyl transferase
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Alt nameCmR
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GenBank IDAY597271
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank Accession #AY597271
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFCM1 was a gift from Herbert Schweizer (Addgene plasmid # 64948 ; http://n2t.net/addgene:64948 ; RRID:Addgene_64948) -
For your References section:
A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923
