pFLP3
(Plasmid #64946)

• Purpose: Flp recombinase expression vector
• Depositing Lab: Herbert Schweizer
• Publication: Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Backbone

• Vector backbone: pFLP1
• Backbone manufacturer: Herbert Schweizer
• Total vector size (bp): 10925
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Flp recombinase

Resource Information

• Supplemental Documents:
  • AY597273
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

GenBank Accession # AY597273

Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown.