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Addgene

SEPTOPO
(Plasmid #64944)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64944 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR4-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3956
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SEP
  • Alt name
    Super Ecliptic pHluorin
  • Species
    Synthetic
  • Insert Size (bp)
    700
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer M13 R
  • 3′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the coding sequence for SEP is lacking a start codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SEPTOPO was a gift from Jeremy Henley (Addgene plasmid # 64944 ; http://n2t.net/addgene:64944 ; RRID:Addgene_64944)

  • For your References section:

    Distinct subunit-specific alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking mechanisms in cultured cortical and hippocampal neurons in response to oxygen and glucose deprivation. Blanco-Suarez E, Hanley JG. J Biol Chem. 2014 Feb 21;289(8):4644-51. doi: 10.1074/jbc.M113.533182. Epub 2014 Jan 8. 10.1074/jbc.M113.533182 PubMed 24403083
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