pcDNA3-SEP-Glur2 (MCA10)
(Plasmid #64941)

• Purpose: mammalian expression of rat GluR2
• Depositing Lab: Jeremy Henley
• Publication: Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

Backbone

• Vector backbone: pcDNA3
• Total vector size (bp): 8852
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GluR2
• Alt name: glutamate receptor, ionotropic, AMPA 2
• Species: R. norvegicus (rat)
• Entrez Gene: Gria2 (a.k.a. GluA2, GluR-K2, GluR2, gluR-B)
• Promoter: CMV
• Tag / Fusion Protein:
  • SEP (superecliptic pHluorin) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F, GFP-F
• 3′ sequencing primer: BGH Reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry