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Addgene

pcDNA3-SEP-Glur2 (MCA10)
(Plasmid #64941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Total vector size (bp) 8852
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GluR2
  • Alt name
    glutamate receptor, ionotropic, AMPA 2
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gria2 (a.k.a. GluA2, GluR-K2, GluR2, gluR-B)
  • Promoter CMV
  • Tag / Fusion Protein
    • SEP (superecliptic pHluorin) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F, GFP-F
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-SEP-Glur2 (MCA10) was a gift from Jeremy Henley (Addgene plasmid # 64941 ; http://n2t.net/addgene:64941 ; RRID:Addgene_64941)

  • For your References section:

    Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs. Ashby MC, De La Rue SA, Ralph GS, Uney J, Collingridge GL, Henley JM. J Neurosci. 2004 Jun 2;24(22):5172-6. 10.1523/JNEUROSCI.1042-04.2004 PubMed 15175386

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