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Addgene

pET3a RegIIIg
(Plasmid #64936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RegIIIg
  • Alt name
    Reg3g
  • Alt name
    regenerating islet-derived 3 gamma
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    417
  • Mutation
    processed form
  • Entrez Gene
    Reg3g (a.k.a. AI449515, REG-3-gamma, reg III-gamma)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a RegIIIg was a gift from Lora Hooper (Addgene plasmid # 64936 ; http://n2t.net/addgene:64936 ; RRID:Addgene_64936)

  • For your References section:

    Regulation of C-type lectin antimicrobial activity by a flexible N-terminal prosegment. Mukherjee S, Partch CL, Lehotzky RE, Whitham CV, Chu H, Bevins CL, Gardner KH, Hooper LV. J Biol Chem. 2009 Feb 20;284(8):4881-8. doi: 10.1074/jbc.M808077200. Epub 2008 Dec 18. 10.1074/jbc.M808077200 PubMed 19095652
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