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pET3a pro-hRegIIIa
(Plasmid #64935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RegIIIa
  • Alt name
    HIP/PAP
  • Alt name
    regenerating islet-derived 3 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    474
  • Mutation
    mutations made in 5' end to relax secondary structure.
  • Entrez Gene
    REG3A (a.k.a. HIP, HIP/PAP, INGAP, PAP, PAP-H, PAP1, PBCGF, REG-III, REG3)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BclI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a pro-hRegIIIa was a gift from Lora Hooper (Addgene plasmid # 64935 ; http://n2t.net/addgene:64935 ; RRID:Addgene_64935)

  • For your References section:

    Refolding, purification, and characterization of human and murine RegIII proteins expressed in Escherichia coli. Cash HL, Whitham CV, Hooper LV. Protein Expr Purif. 2006 Jul;48(1):151-9. Epub 2006 Feb 13. 10.1016/j.pep.2006.01.014 PubMed 16504538
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