pET3a pro-RegIIIg
(Plasmid
#64934)
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Purposebacterial expression of full length mouse RegIIIgamma
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET3a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4640
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRegIIIg
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Alt nameReg3g
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Alt nameregenerating islet-derived 3 gamma
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SpeciesM. musculus (mouse)
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Insert Size (bp)474
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Entrez GeneReg3g (a.k.a. AI449515, REG-3-gamma, reg III-gamma)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET3a pro-RegIIIg was a gift from Lora Hooper (Addgene plasmid # 64934 ; http://n2t.net/addgene:64934 ; RRID:Addgene_64934) -
For your References section:
Refolding, purification, and characterization of human and murine RegIII proteins expressed in Escherichia coli. Cash HL, Whitham CV, Hooper LV. Protein Expr Purif. 2006 Jul;48(1):151-9. Epub 2006 Feb 13. 10.1016/j.pep.2006.01.014 PubMed 16504538