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MSCV-EVA1ext-hFcIgG3-IRES-YFP
(Plasmid #64926)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIY II
  • Backbone manufacturer
    Addgene plasmid 52108
  • Backbone size w/o insert (bp) 6328
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse EVA1 fused with human FcIgG3
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    1145
  • Entrez Gene
    Mpzl2 (a.k.a. Eva, Eva1)
  • Entrez Gene
    IGHG3 (a.k.a. IgG3)
  • Promoter LTR
  • Tag / Fusion Protein
    • IRES-YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN-5
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Was cloned from cDNA from Dr Pflucker lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-EVA1ext-hFcIgG3-IRES-YFP was a gift from Pau Serra (Addgene plasmid # 64926 ; http://n2t.net/addgene:64926 ; RRID:Addgene_64926)

  • For your References section:

    A monoclonal antibody against the extracellular domain of mouse and human epithelial V-like antigen 1 reveals a restricted expression pattern among CD4- CD8- thymocytes. Garabatos N, Blanco J, Fandos C, Lopez E, Santamaria P, Ruiz A, Perez-Vidakovics ML, Benveniste P, Galkin O, Zuniga-Pflucker JC, Serra P. Monoclon Antib Immunodiagn Immunother. 2014 Oct;33(5):305-11. doi: 10.1089/mab.2014.0030. 10.1089/mab.2014.0030 PubMed 25357997
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