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pT7-V5-SBP-C1-HshnRNPE1
(Plasmid #64921)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4229
  • Total vector size (bp) 5300
  • Modifications to backbone
    exchange of the EGFP tag by a V5-SBP tag and insertion of T7 promoter
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hnRNPE1
  • Alt name
    poly(rC)-binding protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1071
  • GenBank ID
    NM_006196.3
  • Entrez Gene
    PCBP1 (a.k.a. HEL-S-85, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 and SBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-V5-SBP-C1-HshnRNPE1 was a gift from Elisa Izaurralde (Addgene plasmid # 64921 ; http://n2t.net/addgene:64921 ; RRID:Addgene_64921)
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